Comparative electrochemical behaviour of biotin hydrazide and photobiotin.Importance in the development of biosensors

The cyclic voltammetric behaviour of biotin hydrazide and photobiotin on ca rbon paste electrodes has been studied. Biotin hydrazide presents an anodic and irreversible process, meanwhile photobiotin presents two, adsorptive i n nature. This characteristic makes photobiotin desirable for following the interaction between biotin and streptavidin, being possible to detect a st reptavidin concentration of 10(-12) M. The evidence of this reaction has be en shown either directly in solution or on the electrode surface. Photobiot in as the molecule portable of analytical information and carbon paste as t he solid support could be applied to the development of sensors based on th e oxidation of this molecule. (C) 1999 Elsevier Science S.A. All rights res erved.