Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell. Lines and a trophoblast/choriocarcinoma cell hybrid

We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells wit h the HGPRT-defective choriocarcinoma cell line ACI-I. The karyotypes of th e parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clon es AC1-1, ACZ-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1 P are presented, together with a detailed characterization of the AC1-speci fic chromosomal marker add(X)(q26) using conventional cytogenetic banding t echniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell h ybrid of trophoblastic origin, providing a unique cell culture model to stu dy trophoblast-related invasion and ifs underlying genetic mechanisms. (C) Elsevier Science Inc., 1999. All rights reserved.