Separation of proteins by electrophoretic affinity chromatography

A new kind of electrophoretic affinity chromatography (EAC) for bioseparati on was proposed. Separation by EAC was conducted in a multicompartment elec trolyzer in which the affinity gel media, were packed in one of the central compartments. The presence of an electric field accelerated the migration of proteins inside the gel matrix during adsorption and desorption processe s. This led to the increase of the overall speed of separation. The present study was focused on the effect of the strength of the electric field on a dsorption and desorption processes.