Effect of Astragalin on matrix secretion and beta 1 integrin mRNA expression in human mesangial cells

Objective To investigate the effect of Astragalin on human renal mesangial cells. Methods Cultured human mesangial cells were treated with Astragalin and Ast ragalin serum in different concentrations in the presence or absence of PDG F-BB, the proliferation and type IV collagen secretion of mesangial cells w ere measured by MTT assay and ELISA, and expression of beta 1 integrin gene was estimated by reverse transcription-polymerase chain reaction (RT-PCR) method, respectively. Results After 72 hours Astragalin or Astragalin serum treatment, the prolif eration of mesangial cells induced by PDGF-BB was inhibited significantly i n a dose-dependent manner compared with untreated controls (P < 0.05 and P < 0.01). After 24 hours of Astragalin or Astragalin serum treatment, the se cretion of type RV collagen protein in presence of PDGF-BB was significantl y decreased and beta 1 integrin mRNA level decreased significantly compared with untreated control (P < 0.05, P < 0.01). Conclusions Astragalin inhibits cell proliferation and matrix over-synthesi s which might be mediated, at least, partly by decrease of beta 1 integrin gene overexpression. The study suggested that Astragalin might play a role in preventing the progression of chronic renal diseases.