Stromal progenitor cells present within liposuction and reduction abdominoplasty fat for autologous transfer to aged skin

BACKGROUND. Autologous fat is used for direct transfer to locally replace f at,las well as for use intradermally in the treatment of rhytids in: aged s kin. OBJECTIVE. TO determine if the components of the autologous fat could be se parated to produce a better agent for the treatment of rhytids, METHODS. Liposuction material from four patients and fat from abdominoplast y from five patients was processed by homogenization and centrifugation to separate mature lipocytes from other stromal cell populations in its associ ated extracellular matrix,:and then to separate this from the blood and cel lular debris. The Cellular layer was evaluated histologically and with the immunohistochemical antibodies for CD34, SM-actin, S-100 protein, MIB-1; Bc l-2, and factor XIIIa. RESULTS. The cellular layer showed spindle cells, some small vascular struc tures, a small amount of mature fat, and extracellular matrix. CD34 showed diffuse staining of most spindle and endothelial cells in all sections, fac tor XIIIa showed only focal staining of spindle and dendritic cells, and Bc l-2 showed light to moderate staining in scattered cells within the cellula r component. S-100 protein, SM-actin, and MIB-1 were negative. CONCLUSION. Processed liposuction and abdominoplasty processed fat map be i deal for the treatment of wrinkles in aged skin because it contains progeni tor stromal cells that are known to be present within the fat and which may be concentrated by processing methods.