During hair follicle (HF) morphogenesis, p75 neurotrophin receptor (p75NTR)
reportedly is the first growth factor receptor found to be expressed by th
ose fibroblasts that later develop into the dermal papilla (DP) of the HF.
However, the functional role of p75NTR in HE morphogenesis is still unknown
. Studying HE development in fetal and neonatal C57BL/6 murine back skin, w
e show that p75NTR-immunoreactivity (IR) is prominently expressed by DP fib
roblasts as well as by skin nerves during the early steps of HF development
. In contrast, p75NTR-IR disappears from the DP in the fully developed HP a
nd it is expressed only in the epithelial outer root sheath of the HF. Comp
ared to age-matched wild-type animals, p75NTR knockout (-/-) mice show sign
ificant acceleration of HF morphogenesis, and DP fibroblasts of p75NTR knoc
kout mice show reduced proliferative activity in situ, indicating alteratio
ns in their transition from proliferation to differentiation. Although no s
ignificant differences in the expression of adhesion molecules (NCAM), sele
cted morphogens (TGF beta-2, HGF/SE, FGF-2, KGF), or their receptors (TGF b
eta R-II, m-met, FGFR-1) were seen between DP of p75NTR knockout and wild-t
ype mice, p75NTR mutants showed a prominent upregulation of FGFR-2, a high-
affinity receptor for KGF, in both follicular DP and epithelium. furthermor
e, the administration of anti-KGF neutralizing antibody significantly inhib
ited acceleration of HF morphogenesis in p75NTR knockout mice in vivo. Thes
e observations suggest that p75NTR plays an important role during HE morpho
genesis, functioning as a receptor that negatively controls HF development,
most likely via alterations in DP fibroblast proliferation/differentiation
and via downregulation of KGF/FGFR-2 signaling in the HF. (C) 1999 Academi
c Press.