Immunoreactivity of the monoclonal antibody F89/160.1.5 for human prion protein

Monoclonal antibodies (Mab) to the prion protein (PrP) have been critical t o the neuropathological characterisation of PrP-related diseases in human a nd animals. Although PrP is highly evolutionary conserved, there is some se quence divergence among species. We have analysed the F89/160.1.5 Mab raise d against the bovine prion protein for immunoreactivity with the human prio n protein. The antibody recognised the IHFG epitope of the prion protein. A n analysis of the Swiss Prot database confirmed conservation of the epitope in humans. Further immunohistochemical (IHC) analysis showed a highly sens itive (final concentration 55 ng/ml) and specific antibody for prion detect ion in humans. The observed immunoreactivity of the prion protein did not d iffer from that observed after staining with the well-known 3F4 (Senetek) m onoclonal antibody.