PROTECTION OF EBSELEN AGAINST ANOXIC DAMA GE OF CULTURED NEURONS OF CEREBRAL-CORTEX

AIM: To study the protective effect of ebselen on anoxic damage of bra in cells. METHODS: On d 10 after plating of the cortical neurons from 1-d-old rat, cultures were placed under 95 % N-2 + 5 % CO2 for 2 - 6 h . Lactate dehydrogenase (LDH) in supernatant, thiobarbituric acid reac tive substance (TBARS) and glutathione peroxidase (GSH-Px) activity of neurons were determined. RESULTS: Under anoxia, efflux of LDH and TEA BS from cultured neurons increased while GSH-Px activity decreased. Eb selen reduced the efflux of LDH and TEARS in a dose-related manner and increased the total GSH-Px activity. CONCLUSION: Ebselen can protect neurons from anoxic damage.