Determination of infectious progeny virus and in vivo labelling with [S-35]
methionine followed by immunoprecipitation demonstrates that the major rece
ptor group human rhinovirus HRV14 is able to infect HeLa cells in the prese
nce of the V-ATPase inhibitor bafilomycin A1. However, host cell shut off i
s delayed and viral yield is decreased in the presence of the drug. Uncoati
ng can thus take place under conditions that prevent endosomal acidificatio
n indicating that it is catalysed by the viral receptor alone. Since transp
ort is arrested in early endosomes upon inhibition of:vesicle acidification
, the data also suggest that productive uncoating takes place from early en
docytic compartments, (C) 1999 Federation of European Biochemical Societies
.