KINETIC-ANALYSIS OF INHIBITOR ACTIONS ON ENZYMES

A set of data on a given peroxidase inhibition by quecertin, showing a n unusually high experimental error, was used to demonstrate how data, seemingly unsuitable for graphical analysis, may still provide useful information on the inhibition mechanism. The most reliable model turn ed out to be a mixed non-competitive inhibition. The present statistic al procedure has proved (i) to be a simple, general and unequivocal wa y to carry on kinetic analysis of enzymatic reactions under inhibition action, based only on consecutive linear regressions (no previous ass umption about the inhibition mechanism is required); (ii) that experim ental errors can play an essential role on the final decision about th e inhibition mechanism and (iii) to be able to show how close to the M ichaelis-Menten mechanism the kinetic model actually is. Therefore all proposed inhibition mechanisms were subjected to statistical judgemen t.