KINETIC-ANALYSIS OF INHIBITOR ACTIONS ON ENZYMES

Citation
W. Brune et al., KINETIC-ANALYSIS OF INHIBITOR ACTIONS ON ENZYMES, Pesquisa agropecuaria brasileira, 32(5), 1997, pp. 457-464
Citations number
19
Categorie Soggetti
Agriculture,"Agriculture Dairy & AnumalScience
ISSN journal
0100204X
Volume
32
Issue
5
Year of publication
1997
Pages
457 - 464
Database
ISI
SICI code
0100-204X(1997)32:5<457:KOIAOE>2.0.ZU;2-1
Abstract
A set of data on a given peroxidase inhibition by quecertin, showing a n unusually high experimental error, was used to demonstrate how data, seemingly unsuitable for graphical analysis, may still provide useful information on the inhibition mechanism. The most reliable model turn ed out to be a mixed non-competitive inhibition. The present statistic al procedure has proved (i) to be a simple, general and unequivocal wa y to carry on kinetic analysis of enzymatic reactions under inhibition action, based only on consecutive linear regressions (no previous ass umption about the inhibition mechanism is required); (ii) that experim ental errors can play an essential role on the final decision about th e inhibition mechanism and (iii) to be able to show how close to the M ichaelis-Menten mechanism the kinetic model actually is. Therefore all proposed inhibition mechanisms were subjected to statistical judgemen t.