Improved plant regeneration from suspension-cultured cells of Iris germanica L. 'Skating Party'

To improve the efficiency of iris plant regeneration, we tested the influen ce of several combinations of Kin and NAA in culture media on the induction of morphogenesis and the subsequent development of plantlets. The highest rates of regeneration (67%) were consistently observed in induction media c ontaining 0.5 mu M NAA and either 2.5 or 12.5 mu M Kin. Developing medium c ontaining 1.25 mu M BA was optimal for high regeneration rates and a high p ercentage of plantlets simultaneously developing shoots and roots. Rooted p lantlets were easily acclimatized and transplanted to various soil mixtures , then grown in the greenhouse. Under optimal conditions as many as 8000 pl antlets could be regenerated from 1 g of cells in approximate to 4 months. Chemical names used: kinetin (Kin); 1-naphthaleneacetic acid (NAA); N-6-ben zyladenine (BA).