Smooth muscle alpha actin and myosin heavy chain expression in the vascular smooth muscle cells surrounding human endometrial arterioles

Endometrial spiral arterioles are believed to play a major role in controll ing menstruation. These arterioles coil and grow through the secretory stag es of the cycle, unlike the 'straight' endometrial arterioles that remain u ncoiled, We postulate that alterations in the growth and development of spi ral arterioles, in particular the vascular smooth muscle cells (VSMC), may contribute to menorrhagia. We examined smooth muscle alpha actin (alpha SMA ) and myosin heavy chains (MHC), two VSMC differentiation markers, in the e ndometrial arterioles of 64 women, comparing them in controls, menorrhagic tissues and across the menstrual cycle. aSMA and MHC expression were determ ined immunohistochemically then evaluated using computer-aided image analys is. alpha SMA expression in the straight arterioles of menorrhagic women wa s reduced in the early secretory stage of the cycle and significantly decre ased at the mid-secretory stage of the cycle (0.67 +/- 0.03 versus 0.55 +/- 0.04, P less than or equal to 0.05), No other significant differences were observed in aSMA and MHC expression in straight arterioles, MHC expression was significantly reduced in the spiral arterioles of menorrhagic tissues at the early secretory stage (0.57 +/- 0.01 versus 0.38 +/- 0.04, P less th an or equal to 0.05), Our results demonstrate differences in the VSMC of me norrhagic women compared with controls, with delayed MNC expression in the spiral arterioles and reduced alpha SMA expression in straight arterioles d uring the mid-secretory stage of the cycle.