Comparison of capsular antigens of Pasteurella multocida strains

The present study was undertaken to compare capsular antigens of Pasteurell a multocida strains isolated from a field case of ovine pneumania (type B) with a reference strain. Capsular antigens were extracted by saline solutio n, potassium thiocyanate and sodium salicylate solution separately. Our of these 3 methods, sodium chloride extraction method resulted in the highest protein concentration in both the strains as compared to other methods. Pro tein profile of both the strains by SDS- PAGE revealed more number of bands by the same method of extraction. Saline solution extract was able to prod uce high antibody titre, whereas sodium salicylate extract was more cross-r eactive.