Evidence of nosocomial Stenothrophomonas maltophilia cross-infection in a neonatology unit analyzed by three molecular typing methods

OBJECTIVE: To characterize the epidemiological relationships among Stenotro phomonas maltophilia isolates in the neonatology unit of our institution ov er a 4-month period in which an increased number of isolates was observed. SETTING: The neonatology ward in a 2,000-bed university hospital in Madrid, Spain. DESIGN: A retrospective molecular epidemiological analysis using three diff erent typing methods, arbitrarily primed polymerase chain reaction (PCR), p ulsed-field gel electrophoresis, and enterobacterial repetitive intergenic consensus-PCR, was performed with 11 isolates obtained from seven neonates over a 4-month period. Presumed unrelated isolates also were included as co ntrols. A similarity dendrogram was obtained, to analyze the genetic relate dness among the isolates. RESULTS: All isolates from the neonates, except one, showed a remarkably hi gh homology among their typing patterns for the three methods assayed and c lustered in the relatedness dendrogram at 96% similarity. The unrelated str ains selected as controls were unclustered. The index case was considered t o be a newborn who had an S maltophilia isolate from a culture drawn on the day of admission to the neonatology unit and which was included in the clu stered similarity group. CONCLUSIONS: Such a high genetic similarity among the isolates, together wi th the presence of an index case who had been colonized or infected by S ma ltophilia before arrival at our institution, constitutes the first evidence of nosocomial cross-transmission of this microorganism (Infect Control Hos p Epidemiol 1999;20:816-820).