Nucleotide sequencing of a polyurethanase gene (pulA) from Pseudomonas fluorescens

Citation
C. Ruiz et Gt. Howard, Nucleotide sequencing of a polyurethanase gene (pulA) from Pseudomonas fluorescens, INT BIO BIO, 44(2-3), 1999, pp. 127-131
Citations number
20
Categorie Soggetti
Environment/Ecology
Journal title
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
ISSN journal
09648305 → ACNP
Volume
44
Issue
2-3
Year of publication
1999
Pages
127 - 131
Database
ISI
SICI code
0964-8305(199909/10)44:2-3<127:NSOAPG>2.0.ZU;2-E
Abstract
Nucleotide sequencing of a gene (pulA) encoding an extracellular polyuretha nase/esterase from Pseudomonas fluorescens revealed an open reading frame e ncoding a 48 kDa protein of 451 amino acid residues. The deduced polypeptid e sequence contained:a serine-hydrolase consensus sequence GXSXG. The polyp eptide lacked an N-terminal signal peptide, but did contain short region to ward the C-terminus that has been observed in secreted lipases, EXXXGXTFIIG SXGNDXIXGGXGXDXXEXXXGXD. The most closely related proteins to the polyureth anase had amino acid sequence homologies of 71%, 67%, 69%, 67% for lipases from P. fluorescens, and 51% for lipases from Serratia marcescens. (C) 1999 Elsevier Science Ltd. All rights reserved.