Determination of the Phytophthora infestans antigen with the use of an immunoenzyme amperometric sensor

Citation
Ep. Medyantseva et al., Determination of the Phytophthora infestans antigen with the use of an immunoenzyme amperometric sensor, J ANALYT CH, 54(12), 1999, pp. 1147-1151
Citations number
24
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF ANALYTICAL CHEMISTRY
ISSN journal
10619348 → ACNP
Volume
54
Issue
12
Year of publication
1999
Pages
1147 - 1151
Database
ISI
SICI code
1061-9348(199912)54:12<1147:DOTPIA>2.0.ZU;2-G
Abstract
It was proposed to diagnose the PhI potato disease, to assess the severity of the disease in plants, and to timely determine the presence of the PhI a ntigen in the test samples using an immunoenzyme sensor. An increase in the catalytic activity of immobilized cholinesterase in the presence of immune complexes under certain conditions can lower the limits of detection of bi ologically active compounds. The proposed version of enzyme immunoassay is superior to other techniques in the rapidity of determination.