Determination of (-)-quinine and (+)-quinidine by liquid chromatography with diode-laser polarimetric detection

Authors
Sanchez, FG Diaz, AN Pareja, AG Montiel, GC
Citation
Fg. Sanchez et al., Determination of (-)-quinine and (+)-quinidine by liquid chromatography with diode-laser polarimetric detection, J AOAC INT, 82(6), 1999, pp. 1308-1315
Citations number
25
Categorie Soggetti
Agricultural Chemistry
Journal title
JOURNAL OF AOAC INTERNATIONAL
ISSN journal
10603271 → ACNP
Volume
82
Issue
6
Year of publication
1999
Pages
1308 - 1315
Database
ISI
SICI code
1060-3271(199911/12)82:6<1308:DO(A(B>2.0.ZU;2-I
Abstract
High-performance liquid chromatography using a combination of photometric, fluorimetric, and diode-laser polarimetric detectors in series for the dete rmination of (+)-quinidine and (-)-quinine was investigated. An RP-8 revers ed-phase column and methanol-water (80 + 20, v/v) with 0.2% triethylamine a s mobile phase at a flow rate of 1 mL/min were used. A dynamic range of 0-2 00 mu g for (+)-quinidine and (-)-quinine was established, with detection l imits of 17.0 and 16.7 mu g, respectively. An application of this method in spiked rabbit serum was developed.