Determination of propionylpromazine hydrochloride in formulation matrixes using reversed-phase ion-pair small bore liquid chromatography

Propionylpromazine hydrochloride (PPZHCl) has been investigated for use wit h leghold traps to reduce the amount of self-inflicted trauma experienced b y animals restrained by these traps. Three types of PPZHCl formulations mad e with Karo dark syrup, K-Y Jelly, and Vaseline were used in 2 types of tra nquilizer trap devices (TTDs). A reversed-phase ion-pair liquid chromatogra phy (LC) method using a small bore C-18 column was used to: (1) determine t he purity of the PPZHCl material used in these formulations, and (2) to det ermine the resulting PPZHCl content of each formulation. Analyte quantitati on was done using UV absorption at 280 nm. Regression analysis of calibrati on standard solutions indicated a linear and directly proportional relation ship between analyte response and PPZHCl concentration over the range evalu ated. Recovery data from: (I) Vaseline formulations containing 38.8, 16.2, and 8.78% PPZHCl were 104, 92.9, and 90.2%, respectively, (2) Karo dark syr up formulations containing 26.5, 18.1, and 10.3% PPZHCl were 97.7, 99.3, an d 106%, respectively, and (3) K-Y Jelly formulations containing 33.0, 23.5, and 13.4% PPZHCl were 100, 99.4, and 88.7%, respectively. The relative sta ndard deviation (RSD) values from triplicate analysis of these formulations ranged from 0.7 to 6.7%. The PPZHCl content from 9 manufactured TTDs, 3 fo r each formulation type, were analyzed in triplicate and produced RSD value s ranging from 0.7-6.8%. These results indicate that the formulation extrac tion presented could be used to evaluate the PPZHCl content in TTDs prior t o field use. The use of a small bore LC column reduced the amount of solven ts consumed and hazardous waste generated, compared to sample analysis that uses a more conventional analytical LC column.