A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice

The ability to successfully intubate the trachea of mice and control their ventilation is important for longitudinal studies requiring recovery from a nesthesia and repeated pulmonary function measurements or other evaluations , such as the use of radiological imaging (e.g., computed tomography or mag netic resonance imaging). We describe a method for rapid and repeated intub ation of mice, with subsequent pulmonary function measurements at baseline and after an agonist challenge. We describe a simply constructed metal blad e used as a laryngoscope to facilitate oropharyngeal exposure, transillumin ation of the neck to facilitate visualization of the trachea through the or opharynx, readily available polyethylene tubing to intubate the trachea, an d a simple solenoid ventilator to maintain physiological ventilation and as sess respiratory resistance and compliance Brief infusions of acetylcholine through a needle into the jugular vein are used to assess the responsivene ss of the airway smooth muscle.