Effects of amino acid substitutions at conserved and acidic residues within region 1.1 of Escherichia coli sigma(70)

Authors
Bowers, CW McCracken, A Dombroski, AJ
Citation
Cw. Bowers et al., Effects of amino acid substitutions at conserved and acidic residues within region 1.1 of Escherichia coli sigma(70), J BACT, 182(1), 2000, pp. 221-224
Citations number
26
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
182
Issue
1
Year of publication
2000
Pages
221 - 224
Database
ISI
SICI code
0021-9193(200001)182:1<221:EOAASA>2.0.ZU;2-O
Abstract
Amino acid substitutions in Escherichia coli sigma(70) were generated and c haracterized in an analysis of the role of region 1.1 in transcription init iation. Several acidic and conserved residues are tolerant of substitution. However, replacement of aspartic acid 61 with alanine results in inactivit y caused by structural and functional thermolability.