Structural and functional analyses of the secondary cell wall polymer of Bacillus sphaericus CCM 2177 that serves as an S-layer-specific anchor

Authors
Ilk, N Kosma, P Puchberger, M Egelseer, EM Mayer, HF Sleytr, UB Sara, M
Citation
N. Ilk et al., Structural and functional analyses of the secondary cell wall polymer of Bacillus sphaericus CCM 2177 that serves as an S-layer-specific anchor, J BACT, 181(24), 1999, pp. 7643-7646
Citations number
29
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
24
Year of publication
1999
Pages
7643 - 7646
Database
ISI
SICI code
0021-9193(199912)181:24<7643:SAFAOT>2.0.ZU;2-C
Abstract
Sacculi of Bacillus sphaericus CCM 2177 contain a secondary cell wall polym er which was completely extracted with 48% hydrofluoric acid. Nuclear magne tic resonance analysis showed that the polymer is composed of repeating uni ts, as follows: --> 3)- [4,6-O-(1-carboxyethylidene)](similar to 0.5)-beta- D-ManpNAc-(1 --> 4)-beta-D-GlcpNAc-(1 --> . The N-terminal part of the S-la yer protein carrying S-layer homologous motifs recognizes this polymer as a binding site.