Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

Authors
Manabe, YC Chen, JM Ko, CG Chen, P Bishai, WR
Citation
Yc. Manabe et al., Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue, J BACT, 181(24), 1999, pp. 7629-7633
Citations number
21
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
24
Year of publication
1999
Pages
7629 - 7633
Database
ISI
SICI code
0021-9193(199912)181:24<7629:CSFEUT>2.0.ZU;2-U
Abstract
A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein ge l analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate inductio n of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.