F. Kannenberg et al., Aberrant oxidation of the cholesterol side chain in bile acid synthesis ofsterol carrier protein-2/sterol carrier protein-x knockout mice, J BIOL CHEM, 274(50), 1999, pp. 35455-35460
Peroxisomal beta-oxidation plays an important role in the metabolism of a w
ide range of substrates, including various fatty acids and the steroid side
chain in bile acid synthesis. Two distinct thiolases have been implicated
to function in peroxisomal beta-oxidation: the long known 41-kDa beta-ketot
hiolase identified by Hashimoto and coworkers (Hijikata, M,, Ishii, N,, Kag
amiyama, H., Osumi, T,, and Hashimoto, T, (1987) J, BioE, Chem, 262, 8151-8
158) and the recently discovered 60-kDa SCPx thiolase, that consists of an
N-terminal domain with beta-ketothiolase activity and a C-terminal moiety o
f sterol carrier protein-alpha (SCPS, a lipid carrier or transfer protein),
Recently, gene targeting of the SCP2/SCPx gene has shown in mice that the
SCPx beta-ketothiolase is involved in peroxisomal beta-oxidation of alpha-m
ethyl-branched chain fatty acids like pristanic acid. In our present work w
e have investigated bile acid synthesis in the SCP2/SCPx knockout mice. Spe
cific inhibition of beta-oxidation at the thiolytic cleavage step in bile a
cid synthesis is supported by our finding of pronounced accumulation in bil
e and serum from the knockout mice of 3 alpha,7 alpha,12 alpha-trihydroxy-2
7-nor-5 beta-cholestane-24-one (which is known bile alcohol derivative of t
he cholic acid synthetic intermediate 3 alpha,7 alpha,12 alpha-trihydroxy-2
4-keto-cholestanoyl-coenzyme A). Moreover, these mice have elevated concent
rations of bile acids with shortened side chains (i,e, 23-norcholic acid an
d 23-norchenodeoxycholic acid), which may be produced via alpha- rather tha
n beta-oxidation, Our results demonstrate that the SCPx thiolase is critica
l for beta-oxidation of the steroid side chain in conversion of cholesterol
into bile acids.