T. Kawazu et al., Expression of a bacterial endoglucanase gene in tobacco increases digestibility of its cell wall fibers, J BIOSCI BI, 88(4), 1999, pp. 421-425
Transgenic tobacco plants exhibiting exogenous cellulase activity were prod
uced by introduction of a truncated cellulase (t-EgI) gene from Ruminococcu
s albus. They accumulated about 0.1-0.5% t-EgI of the total soluble protein
s in the cytoplasm. They were normal in morphology and growth. t-EgI expres
sed in the transgenic plants degraded their own cell wall materials after t
he cells were disrupted. The plants expressing the t-egI gene could be used
as "cellulase-encapsulating plants" for livestock and food industries.