Expression of a bacterial endoglucanase gene in tobacco increases digestibility of its cell wall fibers

Citation
T. Kawazu et al., Expression of a bacterial endoglucanase gene in tobacco increases digestibility of its cell wall fibers, J BIOSCI BI, 88(4), 1999, pp. 421-425
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
88
Issue
4
Year of publication
1999
Pages
421 - 425
Database
ISI
SICI code
1389-1723(199910)88:4<421:EOABEG>2.0.ZU;2-V
Abstract
Transgenic tobacco plants exhibiting exogenous cellulase activity were prod uced by introduction of a truncated cellulase (t-EgI) gene from Ruminococcu s albus. They accumulated about 0.1-0.5% t-EgI of the total soluble protein s in the cytoplasm. They were normal in morphology and growth. t-EgI expres sed in the transgenic plants degraded their own cell wall materials after t he cells were disrupted. The plants expressing the t-egI gene could be used as "cellulase-encapsulating plants" for livestock and food industries.