PREPARATION OF POLYPYRROLE COVALENTLY ATTACHED WITH GLUCOSE-OXIDASE AND ITS APPLICATION TO GLUCOSE SENSING

Glucose oxidase (GOD) was covalently attached to polypyrrole having ca rboxyl groups (PPy-COOH), and polypyrrole attached with GOD(PPy-GOD) w as applied to glucose sensing. The PPy-COOH was prepared by oxidative polymerization of 1-(2-cyanoethyl)pyrrole followed by hydrolysis of th e cyano groups to convert them into carboxyl groups. GOD was bound to the PPy-COOH by condensation reaction between carboxyl groups of the P Py-COOH and amino groups of GOD by use of a carbodiimide reagent. The electrode made of the PPy-GOD thus obtained being used, amperometric r esponse to glucose was monitored. The result shows that the PPy-GOD is a promising material for glucose sensor.