Histochemical analysis of cleared mouse embryos expressing beta-galactosidase

An integrated protocol for clearing, de-clearing, and subsequent histochemi cal analysis of beta-galactosidase expression in mid-gestation mouse embryo s is described. As tissue morphology is preserved after clearing and de-cle aring, this protocol permits rapid identification and precise analysis of b eta-galactosidase expression among a large number of samples. Compared to t he conventional cryosectioning and histochemical analysis, the proposed pro tocol saves a considerable amount of time and resources.