A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines

Authors
Spagnuolo-Weaver, M Fuerst, R Campbell, ST Meehan, BM McNeilly, F Adair, B Allan, G
Citation
M. Spagnuolo-weaver et al., A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines, J IMMUNOL M, 230(1-2), 1999, pp. 19-27
Citations number
17
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGICAL METHODS
ISSN journal
00221759 → ACNP
Volume
230
Issue
1-2
Year of publication
1999
Pages
19 - 27
Database
ISI
SICI code
0022-1759(19991119)230:1-2<19:AFRMFT>2.0.ZU;2-E
Abstract
In this paper, we describe a fluorimeter-based, closed-tube, polymerase cha in reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1 alpha (IL1 alpha) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and comp are it to a standard PCR performed in a block-based thermocycler. (C) 1999 Elsevier Science B.V. All rights reserved.