Heterogeneous Helicobacter pylori isolates from H. pylori-infected couplesin Taiwan

Helicobacter pylori strain diversity was investigated in 55 H, pylori serop ositive couples in Taiwan in biopsy samples from the antrum and corpus. Two DNA typing methods were used to characterize 90 isolates from 25 couples. In only 1 of the 25 couples was the same strain colonized from both partner s, Comparison of isolates from 2 sites in each of 40 patients showed that 9 pairs were distinct but might be related, Peptic ulcer occurred in 77.8% o f these 9 patients compared with 29% of 31 patients with the same predomina nt strain in 2 biopsies (P =.025), Random amplified polymorphic DNA and seq uence analyses of 2 closely related isolates from 1 patient support the hyp othesis that development of genetic diversity of H, pylori results from hor izontal genetic exchange during long-term colonization of mixed bacterial p opulations.