A validated analysis of pemoline in urine by HPLC/DAD after solid phase extraction

A rapid bioanalytical method has been developed for the determination of pe moline in urine, using 4-methyl-primidone as the internal standard. The ana lyte was isolated from urine with a C-18 reverse solid-phase extraction col umn and analysed by high performance liquid chromatography with a photo dio de array detector. The method was linear in the studied range of 0.20 to 1. 40 mu g/mL urine. For the standard curve a weighted (1/x(2)) linear regress ion line (y = - 0.006 + 0.976x) was computed and validated (R-2 = 0.998). T he limit of quantitation was 0.04 mu g/mL urine. Recovery studies for the a ccuracy gave a mean percent recovery of 93.9 and a predicted percent recove ry in the range of 91.5 to 96.0. The precision, expressed as coefficient of variation, was in the range of 2.03 to 3.67 mu g/mL for the intraday and f rom 1.96 to 4.35 mu g/mT, for the inter-day. The ANOVA (analysis of varianc e) tables were also given to validate the day to day reproducibility. Appli cation of the method to clinical samples was demonstrated.