Saccharomyces cerevisiae ISU1 and ISU2: Members of a well-conserved gene family for iron-sulfur cluster assembly

Recent studies in bacteria and eukaryotes have led to the identification of several new genes implicated in the biogenesis of iron-sulfur (Fe/S) clust er-containing proteins. This report focuses on two genes of bakers yeast Sa ccharomyces cerevisiae, ISU1 and ISU2, which encode homologues to bacterial IscU and NifU, potential iron-binding or cluster-assembly proteins. As wit h other yeast genes implicated in Fe/S protein assembly, deletion of either ISU1 or ISU2 results in increased accumulation of iron within the mitochon dria, loss of activity of the [4Fe-4S] aconitase enzyme, and suppression of oxidative damage in cells lacking cytosolic copper/zinc superoxide dismuta se. Both genes are induced in strains expressing an activated allele of Aft 1p, the iron-sensing transcription factor, suggesting that they are regulat ed by the iron status of the cell. Immunoblotting studies using an antibody directed against Escherichia coli IscU reveal that both Isu1p and Isu2p ar e localized primarily in the mitochondria and that Isu1p is the predominant form expressed under all growth conditions tested. The possible role of th e Isu proteins in the assembly and/or repair of Fe/S clusters is discussed. (C) 1999 Academic Press.