The role of abscisic acid in induction of androgenesis: A comparative study between Hordeum vulgare L-cvs. Igri and Digger

Citation
S. Van Bergen et al., The role of abscisic acid in induction of androgenesis: A comparative study between Hordeum vulgare L-cvs. Igri and Digger, J PL GR REG, 18(3), 1999, pp. 135-143
Citations number
29
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
JOURNAL OF PLANT GROWTH REGULATION
ISSN journal
07217595 → ACNP
Volume
18
Issue
3
Year of publication
1999
Pages
135 - 143
Database
ISI
SICI code
0721-7595(199922)18:3<135:TROAAI>2.0.ZU;2-M
Abstract
Under the same mannitol pretreatment and culture conditions, regeneration e fficiency in the barley cultivar. (cv.) Igri was about 10 times higher than in the cv. Digger, a difference only partially reflected by a difference,i n viable microspores after anther pretreatment. Therefore, a comparative st udy between cvs. Igri and Digger was carried out under various pretreatment conditions. For both cultivars, under water, CPW buffer and mannitol pretr eatment conditions, there was a positive correlation between microspore via bility and regeneration efficiency in that mannitol > CPW buffer >> water. Mannitol pretreatment of cv. Igri produced a much higher endogenous abscisi c acid (ABA) level than as to Digger. Addition of ABA stimulated both perce ntages of viability and regeneration efficiency except in the case of manni tol pretreatment. Under CPW buffer pretreatment conditions, addition of ABA significantly stimulated regeneration efficiency and was ABA concentration dependent. However, cv. Digger was less responsive to ABA than cv. Igri. I n both cultivars, under less optimal pretreatment conditions (e.g., water a nd CPW buffer), the effect of ABA was to stimulate increased percentages of viability and/or to reduce the number of binucleate microspores. Moreover, in cv. Igri, direct culture of anthers for 4 days without pretreatment cau sed an increased number of binucleate microspores compared with microspores with pretreatment for 4 days. These binucleate microspores showed DNA degr adation in the nuclei. However, with mannitol pretreatment binucleate micro spores and DNA fragmentation in the nuclei of microspores was rarely observ ed. On the basis of our observations, we suggest that the difference in reg eneration efficiency:in cv. Igri and cv. Digger is related to the differenc es in endogenous ABA production levels under mannitol pretreatment and resp onsiveness to ABA. One of the effects of ABA is likely due to an inhibition of cell death.