Polymerase chain reaction assay to identify all immature stages of two species of the Anopheles quadrimaculatus sibling species complex (Diptera : Culicidae)

Authors
Rutledge, CR Wesson, DM Meek, CL
Citation
Cr. Rutledge et al., Polymerase chain reaction assay to identify all immature stages of two species of the Anopheles quadrimaculatus sibling species complex (Diptera : Culicidae), J AM MOSQ C, 15(4), 1999, pp. 573-575
Citations number
7
Categorie Soggetti
Entomology/Pest Control
Journal title
JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION
ISSN journal
8756971X → ACNP
Volume
15
Issue
4
Year of publication
1999
Pages
573 - 575
Database
ISI
SICI code
8756-971X(199912)15:4<573:PCRATI>2.0.ZU;2-0
Abstract
A polymerase chain reaction assay that detects differences in the 2nd inter nal transcribed spacer of ribosomal DNA was tested as a means to identify a ll immature stages of 2 species in the Anopheles quadrimaculatus sibling sp ecies complex. The technique was successful in amplifying DNA from eggs, 1s t-4th instars, and pupae of An. quadrimaculatus Say and An. inundatus Reine rt.