Fluorescence in situ hybridization analysis of chromosome 1 abnormalities in hematopoietic disorders: rearrangements of DNA satellite II and new recurrent translocations

Citation
M. Busson-le Coniat et al., Fluorescence in situ hybridization analysis of chromosome 1 abnormalities in hematopoietic disorders: rearrangements of DNA satellite II and new recurrent translocations, LEUKEMIA, 13(12), 1999, pp. 1975-1981
Citations number
24
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
LEUKEMIA
ISSN journal
08876924 → ACNP
Volume
13
Issue
12
Year of publication
1999
Pages
1975 - 1981
Database
ISI
SICI code
0887-6924(199912)13:12<1975:FISHAO>2.0.ZU;2-S
Abstract
Using fluorescence in situ hybridization analysis, breakpoints involving th e long arm of chromosome 1 (Iq) were localized in 36 patients with various hematopoietic disorders and rearrangements of the proximal part of Iq, as a scertained with banding techniques. The breakpoint was localized within the satellite Il(sat II) domain in 14 patients with Various abnormalities, bet ween the sat II domain and the BCL9 locus in eight, between the BCL9 and AR NT loci in two, between sat II and ARNT in two others, and distal to ARNT i n seven. A dicentric chromosome 1 was present in two patients. A high incid ence of heterochromatin heteromorphism of chromosome 1 was present in this series. Two recurrent translocations were identified, t(1;2)(q12;q37) in th ree patients suffering from three different acute leukemia subtypes, and t( 1;16)(q12;q24) in two patients with different diseases. Two patients had ju mping translocations. Most of the rearrangements of Iq were secondary abnor malities, included in complex karyotypes. The roles of methylation, interac tions with the proteins interfering with heterochromatin and possible gene silencing due to heterochromatin rearrangements are discussed.