Fluorescence in situ hybridization analysis of chromosome 1 abnormalities in hematopoietic disorders: rearrangements of DNA satellite II and new recurrent translocations
M. Busson-le Coniat et al., Fluorescence in situ hybridization analysis of chromosome 1 abnormalities in hematopoietic disorders: rearrangements of DNA satellite II and new recurrent translocations, LEUKEMIA, 13(12), 1999, pp. 1975-1981
Using fluorescence in situ hybridization analysis, breakpoints involving th
e long arm of chromosome 1 (Iq) were localized in 36 patients with various
hematopoietic disorders and rearrangements of the proximal part of Iq, as a
scertained with banding techniques. The breakpoint was localized within the
satellite Il(sat II) domain in 14 patients with Various abnormalities, bet
ween the sat II domain and the BCL9 locus in eight, between the BCL9 and AR
NT loci in two, between sat II and ARNT in two others, and distal to ARNT i
n seven. A dicentric chromosome 1 was present in two patients. A high incid
ence of heterochromatin heteromorphism of chromosome 1 was present in this
series. Two recurrent translocations were identified, t(1;2)(q12;q37) in th
ree patients suffering from three different acute leukemia subtypes, and t(
1;16)(q12;q24) in two patients with different diseases. Two patients had ju
mping translocations. Most of the rearrangements of Iq were secondary abnor
malities, included in complex karyotypes. The roles of methylation, interac
tions with the proteins interfering with heterochromatin and possible gene
silencing due to heterochromatin rearrangements are discussed.