Assembly of supported membranes studied by surface plasmon microscopy

A home-made surface plasmon microscopy (SPM) was used in cooperation with s urface plasmon resonance spectroscopy (SPR) to analyze the assembly process of the organized fatty acid LB films. The planar fatty acid multilayers we re deposited on a support by piling up different layers of stearic acid and /or palmitic acid monolayers in different regions, which were possible to b e imaged by SPM in the same vision field. A good contrast was obtained betw een different thickness regions and the numerical values of thickness for d ifferent regions were calculated according to the resonance angles.