Using silane coupling reagents in fabrication of DNA arrays on glass support

Authors
Chen, YL Gan, L Lu, ZH
Citation
Yl. Chen et al., Using silane coupling reagents in fabrication of DNA arrays on glass support, MOL CRYST A, 337, 1999, pp. 477
Citations number
3
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
MOLECULAR CRYSTALS AND LIQUID CRYSTALS SCIENCE AND TECHNOLOGY SECTION A-MOLECULAR CRYSTALS AND LIQUID CRYSTALS
ISSN journal
1058725X → ACNP
Volume
337
Year of publication
1999
Database
ISI
SICI code
1058-725X(1999)337:<477:USCRIF>2.0.ZU;2-7
Abstract
Two silane reagents were used to modify the surface of glass slide. The mod ified surfaces were characterized using X-ray photoelectron spectroscopy (X PS). DNA arrays generated by polymerase chain reaction (PCR) from the genom ic DNA of hepatitis B virus (HBV) were spotted on the slides by capillary t ube. Such slides were applied to detect the HBV DNA fragment by hybridizati on with fluorescence-labeled probe.