Dh. Brown et al., Filamentous growth of Candida albicans in response to physical environmental cues and its regulation by the unique CZF1 gene, MOL MICROB, 34(4), 1999, pp. 651-662
Hyphal growth in the opportunistic fungal pathogen Candida albicans is beli
eved to contribute to the virulence of the organism by promoting penetratio
n of fungal cells into host tissue. In this study, stimulation of hyphal gr
owth by a feature of the physical environment was demonstrated. Specificall
y, growth of cells embedded within a matrix promoted the formation of hypha
e. The CZF1 gene, encoding a putative transcription factor, was shown to be
involved in the regulation of hyphal growth under certain conditions, incl
uding embedded conditions. Ectopic expression of CZF1 in embedded cells pro
moted the rapid formation of hyphae. Elimination of CZF1 and CPH1, encoding
a homologue of the Saccharomyces cerevisiae Ste12p transcription factor, l
ed to a pronounced defect in filamentous growth of embedded cells. Eliminat
ion of CZF1 alone led to a moderate defect in hyphal growth under some cond
itions, including embedded conditions. Hyphal morphogenesis in response to
matrix embedding may occur in the opportunistic pathogen, C. albicans, to p
romote invasion of fungal cells into host tissue.