Background. Clyptosporidium, an important cause of diarrhoea, has been repo
rted worldwide both in immunocompetent and immunocompromised individuals an
d has emerged as a serious public health problem. This study was undertaken
to assess the present status of cryptosporidiosis in children and adults w
ith diarrhoea who attended the Nehru Hospital, Chandigarh which is a tertia
ry care hospital.
Methods. Routine stool examination was done using saline and iodine stained
preparations for various parasites. Modified Ziehl-Neelsen and rapid safra
nin-methylene blue techniques were used to detect Clyptosporidium in 2000 s
tool samples (1645 adults, 355 children) from March to November 1998.
Results. Of the 2000 samples, 205 (10.2%) were positive for various parasit
es. Five (1.4%) children were positive for Clyptosporidium and one child wa
s positive for human immunodeficiency virus. in adults, Cryptosporidiumwas
found in only one patient (0.06%). Giardia lamblia was the commonest parasi
te detected both in adults (4%) and children (15.2%).
Conclusion. The present study highlights the importance of Cryptosporidium
as a cause of diarrhoea, especially in children. Thus, there is a need for
specific staining techniques to detect Cryptosporidium in routine diagnosti
c laboratories.