COMPARISON OF EXTRACTION AND SHIPPING METHODS FOR CYSTS AND JUVENILESOF HETERODERA-GLYCINES

Experiments to determine the effects of extraction techniques and the influence of shipping on extraction of Heterodera glycines life stages gave variable results. Shipping did not significantly affect numbers of nematodes extracted. More second-stage juveniles (J2) were extracte d with Baermann funnels than with an elutriator, probably because incu bation of encysted eggs on the Baermann funnel for 1 week allowed hatc hing to occur. Sieving was more efficient than elutriation for extract ing cysts. Adding air agitation to the water pressure during elutriati on increased extraction efficiency of cysts but not J2. Sample sizes o f 250 cm(3) and 500 cm(3) did not influence extraction efficiency of c ysts; however, sample size did influence extraction of J2.