Evolution of shape complementarity and catalytic efficiency from a primordial antibody template

Authors
Xu, JA Deng, QL Chen, JG Houk, KN Bartek, J Hilvert, D Wilson, IA
Citation
Ja. Xu et al., Evolution of shape complementarity and catalytic efficiency from a primordial antibody template, SCIENCE, 286(5448), 1999, pp. 2345-2348
Citations number
50
Categorie Soggetti
Multidisciplinary,Multidisciplinary,Multidisciplinary
Journal title
SCIENCE
ISSN journal
00368075 → ACNP
Volume
286
Issue
5448
Year of publication
1999
Pages
2345 - 2348
Database
ISI
SICI code
0036-8075(199912)286:5448<2345:EOSCAC>2.0.ZU;2-1
Abstract
The crystal structure of an efficient Diels-Alder antibody catalyst at 1.9 angstrom resolution reveals almost perfect shape complementarity with its t ransition state analog. Comparison with highly related progesterone and Die ls-Alderase antibodies that arose from the same primordial germ Line templa te shows the relatively subtle mutational steps that were able to evolve bo th structural complementarity and catalytic efficiency.