In response to concerns that current risk assessment procedures may not ade
quately reflect the long-term risk of pesticides to non-target aquatic plan
ts, the results of standard toxicity tests, based on Lemna minor, have been
compared with responses seen in alternative tests, based on Myriophyllum s
picatum, a submerged dicotyledon, Lagarosiphon major, a submerged monocotyl
edon, and Glyceria maxima, an emergent monocotyledon. For this purpose, sho
rt-term, glasshouse-based studies were conducted to determine the relative
sensitivity of each species to selected herbicides, while a long-term, outd
oor, mesocosm study is in progress to evaluate the ecological relevance of
glasshouse-generated results. Large differences were observed between the s
ensitivities of these species which indicated that Lemna is not the most se
nsitive test species for all herbicides. Furthermore, results from the meso
cosm experiment revealed long-term effects that cannot be predicted from sh
ort-term tests and may have implications for species viability in subsequen
t seasons. The implications for the selection of appropriate species for us
e in toxicity tests will be discussed.