CULTURED RETINAL PERICYTES STIMULATE IN-VITRO ANGIOGENESIS OF ENDOTHELIAL-CELLS THROUGH SECRETION OF A FIBROBLAST GROWTH FACTOR-LIKE MOLECULE

Authors
WATANABE S MORISAKI N TEZUKA M FUKUDA K UEDA S KOYAMA N YOKOTE K KANZAKI T YOSHIDA S SAITO Y
Citation
S. Watanabe et al., CULTURED RETINAL PERICYTES STIMULATE IN-VITRO ANGIOGENESIS OF ENDOTHELIAL-CELLS THROUGH SECRETION OF A FIBROBLAST GROWTH FACTOR-LIKE MOLECULE, Atherosclerosis, 130(1-2), 1997, pp. 101-107
Citations number
39
Categorie Soggetti
Peripheal Vascular Diseas
Journal title
Atherosclerosis → ACNP
ISSN journal
00219150
Volume
130
Issue
1-2
Year of publication
1997
Pages
101 - 107
Database
ISI
SICI code
0021-9150(1997)130:1-2<101:CRPSIA>2.0.ZU;2-V
Abstract
Interaction between cultured endothelial cells (EC) and pericytes (PC) was studied in vitro to clarify the mechanism of diabetic proliferati ve retinopathy. Conditioned medium (CM) from retinal PC strongly incre ased the proliferation and moderately stimulated migration of retinal EC. Moreover, CM from PC caused stimulation of angiogenesis of retinal EC and umbilical cord vein EC in vitro at the same extent as basic fi broblast growth factor (bFGF). PC also stimulated angiogenesis by EC i n mixed cultures. The angiogenic, proliferative and migration activiti es in CM from PC were inhibited by an antibody to bFGF. These data sug gest that PC play an important role in angiogenesis through secretion of an FGF-like molecule. (C) 1997 Elsevier Science Ireland Ltd.