Real-time PCR-analysis of the cytochrome P4501B1 codon 432-polymorphism

The aim of the present study was to develop and validate a rapid assay for genotyping of CYP1B1 codon 432-polymorphism. The described method is a sing le tube assay and combines both rapid-cycle polymerase chain reaction (PCR) with real-time monitoring by amplification and generation of the melting p rofiles of an allele-specific fluorescent probe. With this method 300 sampl es were analysed from healthy, unrelated Germans. Genotype frequency determ ined for the mu tared allele CYP1B1*2 was 0.40. The results show that genot yping of CYP1B1 codon 432-polymorphism with a real-time fluorescence PCR me thod is a rapid and reliable assay for the analysis of large numbers of sam ples.