The successful microbial immobilisation along with fluidisation are keys to
highly effective microbiological wastewater treatment. The Inverse Three-P
hase Fluidised Bed (TPIFB) is a novel technique proposed for wastewater tre
atment. In the present work a method based on analysis of the factors gover
ning the biofilm formation has been developed for production of biocarriers
for packing in a TPIFB for biodegradation of aniline containing wastewater
. Tests of the treated samples show that the procedure ensures forming of a
steady biofilm. The continuous cultivation of Pseudomonas putuda and Ps. a
eruginosa in TPIFB with aniline as a single substrate provides high biodegr
adation rates and stability of the process of wastewater treatment within o
ne-year-long operation. Electrochemical probe for monitoring and control th
e biofilm growth proves its reliability and efficiency in TPIFB.