My. Pushkareva et al., Increased cell-surface receptor expression on U-937 cells induced by 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine, CANCER IMMU, 48(10), 2000, pp. 569-578
Association of the ether lipid, 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosp
hocholine (ET-18-OCH3) with liposomes (ELL-12) reduces acute toxicity while
maintaining or enhancing anticancer activity in experimental tumor models.
ELL-12 has been shown to induce apoptosis by a cytochrome-c-dependent casp
ase-mediated pathway, which results in proteolytic cleavage of poly(ADP-rib
ose) polymerase and lamins, but the antitumor effects of ET-18-OCH3 or ELL-
12 could result from tumor cell differentiation or activation. Here we comp
ared the effects of ET-18-OCH3 and ELL-12 on the expression of cell-surface
proteins associated with cell differentiation and/or activation in U-937 c
ells. Phorbol 12-myristate 13-acetate and all-trans-retinoic acid, which in
duce differentiation in U-937 cells, up-regulated CD11b (MAC1 alpha-integri
n) and CD82 and down-regulated CD71 (transferrin receptor) in a time- and d
ose-dependent manner. In contrast, ET-18-OCH3 and ELL-12 up-regulated both
CD71 and CD11b and did not have any effect on expression of CD82 in U-937 c
ells, suggesting that the ELL-12 may activate these cells rather than induc
e differentiation. Further evidence of activation was that ET-18-OCH3 and E
LL-12 strongly induced tumor necrosis factor cl production by U-937 cells.