DD3: A new prostate-specific gene, highly overexpressed in prostate cancer

Prostate cancer is the most commonly diagnosed malignancy and the second le ading cause of cancer-related deaths in the Western male population. Despit e the tremendous efforts that have been made to improve the early detection of this disease and to design new treatment modalities, there is still an urgent need for new markers and therapeutic targets for the management of p rostate cancer patients. Using differential display analysis to compare the mRNA expression patterns of normal versus tumor tissue of the human prosta te, we identified a cDNA, DD3, which is highly overexpressed in 53 of 56 pr ostatic tumors in comparison to nonneoplastic prostatic tissue of the same patients. Reverse transcription-PCR analysis using DD3-specific primers ind icated that the expression of DD3 is very prostate specific because no prod uct could be amplified in 18 different normal human tissues studied. Also, in a sampling of other tumor types and a large number of cell lines, no exp ression of DD3 could be detected. Molecular characterization of the DD3 tra nscription unit revealed that alternative splicing and alternative polyaden ylation occur. The fact that no extensive open reading frame could be found suggests that DD3 may function as a noncoding RNA. The DD3 gene was mapped to chromosome 9q21-22, and no homology of DD3 to any gene present in the c omputer databases was found. Our data indicate that DD3 is one of the most prostate cancer-specific genes yet described, and this makes DD3 a promisin g marker for the early diagnosis of prostate cancer and provides a powerful tool for the development of new treatment strategies for prostate cancer p atients.