Cardiac troponin I assays for Axsym (Abbott Diagnostics, Abbott Path, IL, U
SA) and Immune 1 (Bayer Corporation, Tarrytown, NY, USA) analysers were eva
luated. Heparin plasma or serum could be used for both assays. Samples were
stable for 24 h at ambient temperature, 3 days at 4-8 degrees C and 3 mont
hs at -20 degrees C. After 10 months' storage at -80 degrees C, the recover
ies were well above 100% by both assays. Total coefficients of variation fo
r Axsym assay were 9.0 %, 5.8 % and 5.3 % at concentrations of 2.6 mu g/l,
9.83 mu g/l and 34.3 mu g/l respectively; for Immune 1 these were 4.4 %, 1.
6 % and 1.8 % at 2.3 mu g/l,6.27 mu g/l and 44.35 mu g/l respectively. It w
as greater than or equal to 20 % at concentration of < 0.5 mu g/l for Axysm
assay and less than or equal to 0.15 mu g/l for Immune 1 assay. Recoveries
were less than or equal to 90 % at less than or equal to 0.22 mu g/l on Ax
sym and at less than or equal to 1.47 mu g/l on Immune 1. Neither method sh
owed significant interference with haemoglobin, bilirubin, triglycerides or
rheumatoid factor. Correlation between the two methods was excellent (r =
0.997, Y (Axsym) = 4.2X (Immuno 1) +3.2). The highest concentrations detect
ed in 50 healthy subjects were 0.3 mu g/l and 0.1 mu g/l by Axsym and Immun
e 1 methods, respectively. Twelve out of 43 renal failure patients had trop
onin I 0.13-0.9 mu g/l using Axsym method and 4 had levels of 0.07-0.13 mu
g/l using Immune 1. In muscle trauma patients, troponin I was undefectable.