Amperometric glucose sensor eased on a polydimethyl siloxane/enzyme-bilayer membrane

An amperometric glucose-sensing electrode based on the cathodic detection o f oxygen consumption during the glucose oxidase (GOx) reaction was prepared as follows. A platinum electrode was modified with polydimethylsiloxane (P DMS), and a GOx-layer was placed on the PDMS layer. The permselectivity of PMDS made it possible to monitor the oxygen consumption rate without interf erence from the hydrogen peroxide. The concentration of glucose (0.01-1.2 m M) could be determined from the decrease in the cathodic current at -0.1 V vs. Ag/AgCl, without any interference from uric acid, acetaminophen and L-c ysteine.