Bacterial lipopolysaccharide-induced inflammation compromises testicular function at multiple levels in vivo

While it is well known that serious illness and inflammation reduce male fe rtility, the mechanisms involved are poorly understood. In adult male rats, a single injection of Lipopolysaccharide at doses that induced either mild or severe inflammation, caused a biphasic decline in Leydig cell testoster one production and gonadotropin responsiveness. In the high dose group only , serum LH levels also were reduced; however, intratesticular testosterone concentrations remained at a level adequate to support qualitatively normal spermatogenesis in both treatment groups. Testicular interstitial fluid fo rmation also declined in a dose-dependent fashion after lipopolysaccharide treatment. In the high dose group only, these hormonal and vascular changes were accompanied by an increase in endothelial permeability, microhemorrha ge, and inflammatory cells in the testis, followed by vacuolization of roun d spermatid nuclei, disruption of Sertoli-germ cell contacts at stages I-IV of the cycle of the seminiferous epithelium, and subsequently apoptosis of spermatocytes at stages II-V. These data indicate that mild inflammation c auses local inhibition of Leydig cell function with relatively little sperm atogenic damage. The pathological changes in spermatogenic function during severe inflammation are most likely due to direct effects of inflammatory m ediators on the seminiferous epithelium or testicular vasculature, rather t han inhibition of the brain-pituitary-leydig cell axis.