A. Contreras et al., Influence of sparger on energy dissipation, shear rate, and mass transfer to sea water in a concentric-tube airlift bioreactor, ENZYME MICR, 25(10), 1999, pp. 820-830
Data on volumetric mass-transfer coefficient. K-L alpha L, in a 12 X 10(-3)
m(3) airlift bioreactor are reported. Measurements were made in sea water.
The superficial gas velocity ranged up to 0.21 m/s. Four cylindrical sparg
ers (60-1000 mu m pore size) were tested. In bubbly flow, the sparger pore
size strongly influenced the K(L)a(L); the highest K(L)a(L) values were obt
ained with the smallest pore size. Ln contrast, in the transition and heter
ogeneous flow regimes, the pore size had little influence on K,a,. The best
correlation of the mass transfer data was obtained when both gas holdup an
d liquid superficial velocity were taken as independent variables. Shear ra
tes were estimated in the different zones of the reactor. The highest value
s were found in the bottom zone of the reactor and in the gas-liquid separa
tor. The penetration and isotropic turbulence models were used to develop a
semi-theoretical equation relating the volumetric mass-transfer coefficien
t to shear rate; hence providing a better understanding of how the operatio
nal variables may be manipulated to attain a moderate shear rate and an app
ropriate level of mass transfer, two extremely important parameters for the
growth of sensible microorganisms as those used in marine biotechnology. (
C) 1999 Elsevier Science Inc. All rights reserved.