Novel HPLC analysis of tocopherols, tocotrienols, and cholesterol in tissue

Tocopherols and tocotrienols are being increasingly recognized to have an i mportant role in the prevention of atherosclerosis. It has been reported th at they protect low-density Lipoprotein (LDL) and tissues from oxidative st ress and that tocotrienols can reduce plasma cholesterol levels. Two isocra tic high-performance liquid chromatography (HPLC) methods for simultaneous analysis of tocopherols, tocotrienols, and cholesterol in muscle tissue wer e developed. Method A involves basic saponification of the sample, but caus es losses of the gamma- and delta-homologs of vitamin E. Method B does not. involve saponification, thereby protecting the more sensitive homologs. Bo th permit rapid analysis of multiple samples and neither requires specializ ed equipment. These methods may provide techniques useful in simultaneous a ssessment of oxidative stress status (OSS) and cholesterol levels. (C) 1999 Elsevier Science Inc.